1x ebioscience™ 1-step fix Search Results


qscript xlt 1-step rt-qpcr toughmix  (Quanta Biosciences)
96
Quanta Biosciences qscript xlt 1-step rt-qpcr toughmix
Qscript Xlt 1 Step Rt Qpcr Toughmix, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/qScript+XLT+1-Step+RT-qPCR+ToughMix/custom%4095133-100%4038012172
Average 96 stars, based on 1 article reviews
qscript xlt 1-step rt-qpcr toughmix - by Bioz Stars, 2026-10
96/100 stars
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1x 1step rt qpcr probe 4x kit  (highQu Inc)
92
highQu Inc 1x 1step rt qpcr probe 4x kit
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
1x 1step Rt Qpcr Probe 4x Kit, supplied by highQu Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/1Step+RT+qPCR+Probe+Kit/pmc10682382-161-42-49
Average 92 stars, based on 1 article reviews
1x 1step rt qpcr probe 4x kit - by Bioz Stars, 2026-10
92/100 stars
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1x itaq universal sybr green 1 step reaction mix  (Bio-Rad)
96
Bio-Rad 1x itaq universal sybr green 1 step reaction mix
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
1x Itaq Universal Sybr Green 1 Step Reaction Mix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/ITaq+Universal+With+Sybr+Green+In+One+Step/pm26230769-72-9-17
Average 96 stars, based on 1 article reviews
1x itaq universal sybr green 1 step reaction mix - by Bioz Stars, 2026-10
96/100 stars
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1x gotaq 1-step rt-qpcr system  (Promega)
90
Promega 1x gotaq 1-step rt-qpcr system
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
1x Gotaq 1 Step Rt Qpcr System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/gotaq+qpcr+master+mix/pm40006997-54-6-11
Average 90 stars, based on 1 article reviews
1x gotaq 1-step rt-qpcr system - by Bioz Stars, 2026-10
90/100 stars
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1-step rt-qpcr toughmix avantor, radnor, pa  (Avantor)
90
Avantor 1-step rt-qpcr toughmix avantor, radnor, pa
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
1 Step Rt Qpcr Toughmix Avantor, Radnor, Pa, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/1+step+rt+qpcr+toughmix+avantor++radnor++pa/pmc08230961__au1c00048_si_001-271-18-23
Average 90 stars, based on 1 article reviews
1-step rt-qpcr toughmix avantor, radnor, pa - by Bioz Stars, 2026-10
90/100 stars
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goscript™ rt mix for 1-step rt-qpcr (50×)  (Promega)
90
Promega goscript™ rt mix for 1-step rt-qpcr (50×)
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
Goscript™ Rt Mix For 1 Step Rt Qpcr (50×), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/goscripttm+rt+mix+for+1+step+rt+qpcr/pmc07600529-91-23-13
Average 90 stars, based on 1 article reviews
goscript™ rt mix for 1-step rt-qpcr (50×) - by Bioz Stars, 2026-10
90/100 stars
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ultraplex 1-step toughmix  (Quanta Biosciences)
94
Quanta Biosciences ultraplex 1-step toughmix
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
Ultraplex 1 Step Toughmix, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/UltraPlex+1-Step+ToughMix/custom%4095166-500%4032970706
Average 94 stars, based on 1 article reviews
ultraplex 1-step toughmix - by Bioz Stars, 2026-10
94/100 stars
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1-step fix/lyse solution  (Becton Dickinson)
90
Becton Dickinson 1-step fix/lyse solution
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
1 Step Fix/Lyse Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/1+step+fix+lyse+solution/pm37117247-177-6-36
Average 90 stars, based on 1 article reviews
1-step fix/lyse solution - by Bioz Stars, 2026-10
90/100 stars
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primers biolegio, nijmegen, the netherlands  (Biolegio bv)
90
Biolegio bv primers biolegio, nijmegen, the netherlands
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
Primers Biolegio, Nijmegen, The Netherlands, supplied by Biolegio bv, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/specific+primers+biolegio/pmc08230961__au1c00048_si_001-271-2-3
Average 90 stars, based on 1 article reviews
primers biolegio, nijmegen, the netherlands - by Bioz Stars, 2026-10
90/100 stars
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odyssey  (LI-COR)
99
LI-COR odyssey
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
Odyssey, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/Odyssey/custom%40Odyssey%4010%2E1101%2F2020%2E08%2E27%2E270553
Average 99 stars, based on 1 article reviews
odyssey - by Bioz Stars, 2026-10
99/100 stars
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odyssey imaging system  (LI-COR)
99
LI-COR odyssey imaging system
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
Odyssey Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/Odyssey+Imaging+System/custom%40odyssey-imaging-system%4010%2E1101%2F2020%2E08%2E27%2E270553
Average 99 stars, based on 1 article reviews
odyssey imaging system - by Bioz Stars, 2026-10
99/100 stars
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1x clarity™ jn solution  (JN Medsys)
90
JN Medsys 1x clarity™ jn solution
Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by <t>(RT-)qPCR</t> of NA extracted from 100; 1000; 10,000 and 100,000 cells.
1x Clarity™ Jn Solution, supplied by JN Medsys, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+ebioscience%E2%84%A2+1-step+fix/claritytm+jn+solution/med_rxiv__2021__06__10__21258725-57-26-30
Average 90 stars, based on 1 article reviews
1x clarity™ jn solution - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by (RT-)qPCR of NA extracted from 100; 1000; 10,000 and 100,000 cells.

Journal: Scientific Reports

Article Title: NAxtra magnetic nanoparticles for low-cost, efficient isolation of mammalian DNA and RNA

doi: 10.1038/s41598-023-46868-5

Figure Lengend Snippet: Nucleic acid (NA) extraction from 100 to 1,000,000 cells (HAP1), comparing the NAxtra-based method on KingFisher Flex (three independent NA extractions) to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). (A) NAs extracted from 1 = 1,000,000 and 2 = 500,000 cells, separated on a 0.4% agarose gel (left, 1% eluate applied for 1,000,000 cells, 2% eluate applied for 500,000 cells) with GeneRuler High Range DNA ladder (Thermo Scientific), or 1.2% agarose gel (right, 4% eluate applied for 1,000,000 cells, 8% eluate applied for 500,000 cells) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). Gel images have been inverted and cropped; original gels are presented in Supplementary Fig. . ( B ) Average NA yields (± 1 SD) from 500,000 and 1,000,000 cells, as measured by Quant-iT RNA/DNA assay (Invitrogen). ( C ) Average cycle threshold (Ct) values (± 1 SD) for DNA target (left; a 305 bp, single-locus genomic DNA amplicon from the KAPA Human Genomic DNA Quantification and QC Kit) and RNA target (right; ACTB) amplified by (RT-)qPCR of NA extracted from 100; 1000; 10,000 and 100,000 cells.

Article Snippet: ACTB mRNA was reverse transcribed and amplified in reactions of 2.5 μl template RNA, 1X ACTB PrimeTime XL PCR Assay Hs.PT.39a.22214747 (Integrated DNA Technologies), and either 1X qScript XLT 1-Step RT-qPCR ToughMix (Quantabio), 1X One-Step Takyon Ultra Probe 4X MasterMix (Eurogentec), or 1X 1Step RT qPCR Probe 4X Kit (highQu) with annealing at 62 °C and remaining reaction conditions according to master mix specifications.

Techniques: Extraction, Agarose Gel Electrophoresis, Amplification, Quantitative RT-PCR

Nucleic acids (NA) extracted from adherent cells (HAP1) using the NAxtra-based method on KingFisher Flex (three independent NA extractions) compared to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). Average cycle threshold (Ct) values (± 1 SD) for a DNA target (top, MYC) and RNA target (bottom, ACTB) amplified by (RT-)qPCR of NAs extracted from 100 cells and 100,000 cells of HAP1. Two different master mixes were used for each target: RealQ Plus 2 × Master Mix for Probe (Ampliqon) and Luna Universal Probe qPCR Master Mix (NEB), or One-Step Takyon Ultra Probe 4X MasterMix (Eurogentec) and 4X 1Step RT qPCR Probe Kit (highQu).

Journal: Scientific Reports

Article Title: NAxtra magnetic nanoparticles for low-cost, efficient isolation of mammalian DNA and RNA

doi: 10.1038/s41598-023-46868-5

Figure Lengend Snippet: Nucleic acids (NA) extracted from adherent cells (HAP1) using the NAxtra-based method on KingFisher Flex (three independent NA extractions) compared to the AllPrep DNA/RNA/miRNA Universal Kit (two independent NA extractions). Average cycle threshold (Ct) values (± 1 SD) for a DNA target (top, MYC) and RNA target (bottom, ACTB) amplified by (RT-)qPCR of NAs extracted from 100 cells and 100,000 cells of HAP1. Two different master mixes were used for each target: RealQ Plus 2 × Master Mix for Probe (Ampliqon) and Luna Universal Probe qPCR Master Mix (NEB), or One-Step Takyon Ultra Probe 4X MasterMix (Eurogentec) and 4X 1Step RT qPCR Probe Kit (highQu).

Article Snippet: ACTB mRNA was reverse transcribed and amplified in reactions of 2.5 μl template RNA, 1X ACTB PrimeTime XL PCR Assay Hs.PT.39a.22214747 (Integrated DNA Technologies), and either 1X qScript XLT 1-Step RT-qPCR ToughMix (Quantabio), 1X One-Step Takyon Ultra Probe 4X MasterMix (Eurogentec), or 1X 1Step RT qPCR Probe 4X Kit (highQu) with annealing at 62 °C and remaining reaction conditions according to master mix specifications.

Techniques: Amplification, Quantitative RT-PCR

RNA isolated from triplicates of 1,000,000 cells (HAP1) using the NAxtra-based method compared to the MagMAX mir Vana Total RNA isolation kit (Applied Biosystems) on KingFisher Duo Prime. Statistical analysis was performed by two-sided, unpaired t-tests, in which ns (non-significant) = P > 0.05, * = P ≤ 0.05, ** = P ≤ 0.01, and **** P ≤ 0.0001. ( A ) Total RNA separated on a 1.2% agarose gel (5% eluate applied) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). The gel image has been inverted and cropped; the original gel is presented in Supplementary Fig. . ( B ) Average total RNA yield (± 1 SD), as measured by Quant-iT RNA assay (Invitrogen) ( C ) Average cycle threshold (Ct) values (± 1 SD) for five miRNA targets; hsa-miR-24-3p, hsa-miR-122-5p, hsa-miR-210-5p, hsa-miR-455-5p and hsa-miR-1246, amplified by RT-qPCR. D) Average cycle threshold (Ct) values (± 1 SD) for an mRNA target (ACTB) amplified by RT-qPCR.

Journal: Scientific Reports

Article Title: NAxtra magnetic nanoparticles for low-cost, efficient isolation of mammalian DNA and RNA

doi: 10.1038/s41598-023-46868-5

Figure Lengend Snippet: RNA isolated from triplicates of 1,000,000 cells (HAP1) using the NAxtra-based method compared to the MagMAX mir Vana Total RNA isolation kit (Applied Biosystems) on KingFisher Duo Prime. Statistical analysis was performed by two-sided, unpaired t-tests, in which ns (non-significant) = P > 0.05, * = P ≤ 0.05, ** = P ≤ 0.01, and **** P ≤ 0.0001. ( A ) Total RNA separated on a 1.2% agarose gel (5% eluate applied) with GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific). The gel image has been inverted and cropped; the original gel is presented in Supplementary Fig. . ( B ) Average total RNA yield (± 1 SD), as measured by Quant-iT RNA assay (Invitrogen) ( C ) Average cycle threshold (Ct) values (± 1 SD) for five miRNA targets; hsa-miR-24-3p, hsa-miR-122-5p, hsa-miR-210-5p, hsa-miR-455-5p and hsa-miR-1246, amplified by RT-qPCR. D) Average cycle threshold (Ct) values (± 1 SD) for an mRNA target (ACTB) amplified by RT-qPCR.

Article Snippet: ACTB mRNA was reverse transcribed and amplified in reactions of 2.5 μl template RNA, 1X ACTB PrimeTime XL PCR Assay Hs.PT.39a.22214747 (Integrated DNA Technologies), and either 1X qScript XLT 1-Step RT-qPCR ToughMix (Quantabio), 1X One-Step Takyon Ultra Probe 4X MasterMix (Eurogentec), or 1X 1Step RT qPCR Probe 4X Kit (highQu) with annealing at 62 °C and remaining reaction conditions according to master mix specifications.

Techniques: Isolation, Agarose Gel Electrophoresis, Amplification, Quantitative RT-PCR